Corynebacterium glutamicum was chosen as a typical gram-positive model and Nostoc sp. In this work, we present a lysis protocol that can be used to extract genomic DNA from both gram-positive and gram-negative species without interfering with the amplification chemistry. The major challenges that bacterial species pose to genome amplification from single cells include the rigid bacterial cell walls and the need for an effective lysis protocol compatible with microfluidic platforms. So far, single-cell sequencing has been focused mostly on human cells due to the ease of lysing the cells for genome amplification. This technology is mostly coupled with microfluidic systems for controlled cell manipulation and precise fluid handling to shed light on the genomes of a wide range of cells. Single-cell sequencing is a powerful technology that provides the capability of analyzing a single cell within a population.
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